Wind-dispersed pollen is a serious problem for most allergy sufferers. In rats injected with D-galactose (rodent model for premature aging thought to be related to AGE formation) oral ingestion of 500-1,500mg/kg of pine pollen extract is able to hinder AGE formation and symptoms of aging with the higher dose being comparable in potency to 100mg/kg aminoguanidine. It is a native of tropical areas and it is of great economical value to the man as inner white flesh can be eaten and its water is used as a health drink. Visit the Solution Center to Explore Articles, Fairfax County Health Department, Virginia, Register Your Anaphylaxis is when you get severe allergic reactions. If a cockroach reaction is detected, a person might be put on antihistamines or given a nasal spray or eyedrops. Lactase is an enzyme that is in the lining of the gut. The aim of this study was to evaluate, with Brown Norway rats, the allergenic potential of isolated PCGs and to compare it with the allergenicity of whole timothy grass pollen. Pine Pollen Powder: Benefits, Uses & Side EffectsPollen has been considered as one of the reliable sources of medicine for more than 2000 years. When beginning an elimination diet, keep a record of which foods have been removed or added, and the response. ... if you are allergic to kiwi you can be allergic to bird pollen as well and you can be allergic to latex . The free national and local pollen count service offered by Pollen.com enables you to not only see what the pollen count is throughout the nation, but also provides you with a 5-day forecast for your area. Besides poor digestion, grains can affect the tummies and also some terriers are allergic to grains. Keep a record of when each food is added and the response. In delayed hypersensitivity the allergic response occurs only after there has been repeated exposure to a food, or product, or antigen, that has been previously encountered. Seasonal allergies can make life absolutely miserable. https://ratguide.com/health/integumentary_skin/food_allergies.php Bee pollen may also help heal burns. Signs and symptoms are similar to that of eczematous dermatitis, and are like that seen with ectoparasite infestation, or fungal infections. Allergens are everywhere in the world around us. Pollen is famous for it. Allergy immunotherapy (allergy shots) is a process of stimulation of the immune system with gradually increasing doses of the substances to which a person is allergic. I think most allwergic issues with rats would be while being held. Such as the common rat treat called yogies. If no new foods have been added to the diet or the specific food agent is unable to be identified readily, then What Is a Grass Pollen Allergy? Neurology. Keep nails clipped to prevent injury or chance of infection to skin from scratching. One way you may notice that you are dealing with a rodent problem in your home is if you are experiencing strange rashes on your body and you do not know what the cause is, or if you are experiencing allergy symptoms when you know that it is not the season when you normally experience allergies. Smoke Traditional allergy tests utilize the Skin Prick method (also known as a Puncture or Scratch test) to determine whether an allergic reaction will occur by inserting possible triggers into your skin using a needle prick. The purpose of the study was to assess the allergenic potential of washed and unwashed PCGs, from Phleum pratense pollen grains, in the Brown Norway rat, and to study the IgE reactivity of sera of sensitized rats to water-soluble and water-insoluble extracts of PCGs and pollen grains. Allergy pollen counts allow you to see how you could be affected by pollen right in your own hometown or anywhere throughout the nation. Pollen. A study found that in people with grass pollen allergies there was an upregulation of MC1R in their noses. The Rat Guide and its affiliates accept no responsibility for misuse or misunderstanding of its information. Though, contact allergic dermatitis is commonly found and there are some reported cases of sensitization to coconut pollen. Pollen allergen, one of the biological component of PMs, contributes to several health problems, such as asthma, pollinosis, and allergic rhinitis (Butres et al., 2015; Humbal et al., 2018; Fernandez-Gonzalez et al., 2019). Also at those times, especially when your car is covered with pollen, wash the car, put on the air conditioner and drive with the windows closed. Most food allergies in rats are often of a delayed hypersensitivity response. Just like being allergic to cats and dogs, you can also be allergic to rats, mice or guinea pigs. Pet saliva and urine also are sources of allergy-causing proteins, which means when a cat licks itself then gets close to someone, it can be a cause of an allergic reaction. Allergy to Pollen. ? [ ref ] If that gene sounds familiar, it is the same gene that codes for the melanin receptor which causes red hair. Immunotherapy is very effective for management of allergies to pollen, mites, cats, and especially stinging insects (for example, bees). In general, IgE mediated reactions typically show with rapid development of symptoms, where as, in food hypersensitivity non-IgE mediated reactions being cell mediated tend to develop over hours to days; although combined IgE and non-IgE reactions may occur. Inbred Female Brown Norway (BN/NHhsd) rats (n = 18) were obtained from Harlan-Sprague Dawley (Indianapolis, IN). Business, Privacy Food sensitivities can include any type of adverse reaction to a food or food additive and can be divided into categories such as: In food allergies, proteins within the food may be responsible for a reaginic immune mediated antibody-antigen (IgE) response, when there is an immediate reaction. I do take prescription tablets for my allergies but they dont always work if I'm around a dog for example for more than a couple of hrs. If symptoms return following the addition of a particular food then you have identified the culprit food and can remove it from the diet. Rodents are not directly known for being poisonous, but having rodents in a home can indirectly be responsible for a number of heath ailments, especially to individuals who are prone to rat allergies. Like im allergic to mice, rats and insect repellant. Likewise, research studies indicate it may function to stimulate the immune system to produce antibodies that calm an allergic … Female rats tend to have less oily … Seasonal allergic rhinitis, often referred to as hay fever, is typically caused by outdoor allergens such as pollen from trees, grasses and weeds. Allergic reactions are commonly caused by pet dander (dead skin flakes), saliva, and urine. As far as allergies go, the theory is that because bee pollen has demonstrated anti-inflammatory properties in rats, it may be able to counteract the inflammation that occurs in a human’s eyes, nose, or throat during an allergic reaction. Rats produce three types of allergens: dander (dead skin cells), saliva and urine. Of all the pollen, and mold, and animal dander, dust mites that we have studied, the cat dander is absolutely the smallest dander. This can be seen where lactase deficiency is present. Summary. Treat skin lesions, skin irritation, or lesions. Methods: Rats were sensitized (day 0) and challenged (day 21), in controlled comparative conditions, with pollen grains (0.5 mg) or PCGs (4.5 x 10(7) and 0.5 mg). Pollen grains are well established to be an important cause of respiratory allergy. If mice have invaded your home they could be causing an allergic reaction. Center, Investor (Contrary to popular belief, animal fur does not cause allergies.) Find out more. Those whose pet allergies trigger allergic bronchitis or asthma, may also develop the following Respiratory symptoms: • Cough • Wheezing (whistling sound) when breathing ... ☐ If you are deciding between a mouse and a rat, get a mouse, because mice are less likely to aerosolize allergens than rats. Tablets that dissolve under the tongue are now available by prescription for people suffering from grass and ragweed allergies. Rat allergy symptoms may appear immediately if you are prone to rodent droppings allergies. For example, you may have a runny nose if exposed to pollen, develop a rash if you have a skin allergy, or feel sick if you eat something you're allergic to. Going into anaphylactic shock can be life-threatening. You can't get more natural than plants. Time of year for indoor allergens In tests on rats, the study's authors determined that bee pollen may help boost bone levels of calcium and phosphate and protect against osteoporosis-related bone loss. Uggh :l What is yours?? Household pets, including dogs, cats, and gerbils, and farm animals, are a common cause of allergies. Examples of common allergens are pollen, foods, and mold.. True: Allergic rhinitis is the medical term for a group of allergies that typically cause symptoms in the nose, throat, eyes, ears, skin and roof of the mouth. Unfortunately for people with grass allergies, this pollen triggers allergic reactions. They also had a higher hemoglobin content and greater number of red blood cells after pollen consumption. In conclusion, our study demonstrated the ability of A. scholaris pollen extract to induce a Th2-biased allergic response in rats. These scratches can become inflamed and result in hives for the owner, ending in a not-so-wonderful kind of day. Possible Side Effects Serious allergic reactions to bee pollen have been reported, including potentially life … When you have allergies, your immune system makes antibodies that identify a particular allergen as harmful, even though it isn't. The protein found in rat urine can cause itchy rashes on the skin. If your cat suffers from mild discomfort seasonally, your vet may advise to keep your pet indoors in order to limit exposure to pollen. According to the Fairfax County Health Department, Virginia, rodent droppings and urine contain a special type of protein that may cause serious allergic reactions to some people. Apart from rodent droppings allergies, the rodents also serve as a carrier for a number of infectious diseases such as hantavirus, which can be fatal. There are two types of allergic rhinitis. [13] Find out more. Allergy testing is not routinely done in rodents. Cats that suffer from pollen allergies have an immune system deficiency, and the immune system doesn't recognize the pollens and considers them as a threat, so histamines will be produced. All comments are subject to review and approval prior to posting. Not all felines respond negatively to pollens. Acute Stress Promotes Aggressive-Like Behavior in Rats Made Allergic to Tree Pollen. Each year, plants (including grasses) release tiny pollen grains to fertilize other plants of the same species. Saliva, sweat, pee, and dander (dead skin cells) can all trigger allergies. Cats are exposed to various irritants in the environment, including chemicals or pollens. This can sometimes make it hard to discern if food allergies, or another one of these causes is actually responsible for the dermatitis, unless one can identify that parasites are present or knows that an offending food has been given. According to Allergy Matters, a study found that rats cause more skin based allergic reactions as opposed to respiratory issues. Studies have proved that mice and rats fed with pollen showed a higher vitamin C and magnesium content in the thymus, heart muscle and skeletal muscles. Like dogs and cats, other animals like guinea pigs and gerbils can produce indoor allergens in the air—as can mice and rats. "If you have a severe allergic response—for instance people have peanut allergies or egg allergies or food allergies—you can still get this vaccine, you … It is not usually the showy, spring flowering trees that are triggering your allergies. There are no published reports on rodents with inhalant (pollen) allergies. Businesses, Project Cost Pollen, a common allergen, is a substance released by every tree, flower, and weed. Guinea pigs, rabbits, birds, mice and rats can also trigger asthma or allergies in some people. Pollen allergies are fairly common. Find out which animals are the worst offenders when it comes to allergic … This guide in whole or part, exists solely for the purpose of recognizing and understanding the care and illnesses in the pet rat. Objective. A Food Intolerance of unknown or known immunological mechanisms, such as , lactose intolerance. There are tree pollen in spring, herbaceous pollen in summer, and ragweed pollen in autumn. Additionally, when … It is rare for a rat to be allergic to or sensitive to most litters though, beside pine or cedar shavings. If those are the royal jelly benefits you were looking for, bee pollen is a safer alternative. Because of the seasonal variation in your symptoms, … is the co-owner of Rodents Stop, which provides pest control services in Los Angeles. Need professional help with your project? True. Food allergies also are common. Hi, new to group but wondered if anyone had any insights on this, I suffer with asthma and I have allergies to cats/dogs/pollen.. We also own 2 pet rats, always kept in their cage, always clean but I wondered if they could be causing my asthma to worsen but not get the usual allergic reactions that I would to cats/dogs (sneezing ect) According to the Fairfax County Health Department, Virginia, rodent droppings and urine contain a special type of protein that may cause serious allergic reactions to some people. I recently had to get rid of my lovely pet rats because I was so allergic to them, but I miss a furry companion. Enter a zip below and get matched to top-rated pros near you. If mom isn't holding the rat, I doubt that there will be an issue. For instance, releasing histamine from mast cells, induced by … Some of these allergens are dry and flaky; therefore, they can easily become airborne and can travel through your air ducts and settle on your home furnishings or bedroom mattress. Just like being allergic to cats and dogs, you can also be allergic to rats, mice or guinea pigs. Rule out other causes such as parasites, fungal infections, or other disease process such as tumor growth, or metabolic conditions. Allergic rhinitis (AR) is a major concern in personal and public health, which negatively affects emotions and behavior, leading to cognitive deficits, memory decline, poor school performance, anxiety, and depression. Some of those compounds involved are yellow dye number 5, monosodium glutamate, and sulfites which can occur naturally in food or be an additive to prepared foods. Many veterinary practitioners have not had sufficient education in rats … With food intolerance, the immune system is not involved. A Layman's Guide to Health, Medication Use, Breeding, and Responsible Care of Pet Rats. In most parts of the U.S., tree and grass pollen from Spring into Summer, and ragweed and other weed pollen in Fall, are triggers of hay fever or seasonal allergic rhinitis. If you notice symptoms of anaphylaxis, such as having trouble breathing, use an epinephrine injector. Potential Side Effects of Bee Pollen. Some people are allergic to tree pollen, which is in the air in spring. Pollen allergies are usually treated either conservatively or with a variety of prescription medicines, depending on the severity of symptoms. I was wondering if pet rats would be ok for me to have. Rats allergic to pollen were also more aggressive during a social intruder task if they were exposed to both pollen and stress (i.e. Double Rex and sometimes even Rex rats can also be more tolerable for those who have allergies. Culprit foods identified and removed from diet. So while you might very well be allergic to the pines around you, it may rather be the case that some other tree is making you sneeze and you blame the pine because it’s the pollen you can see. Secondly, they have the tendency of picking up urine with their feet and then spreading their urine to other parts of your home. Did you know that avoiding certain foods might relieve your allergy symptoms if you are allergic to grass pollen? For many people, spring is the worst time, but some plants produce pollen in other months of the year. Author information: (1)Laboratory of Behavioral Neuroimmunology, Mood and Anxiety Program, Department of Psychiatry, University of Maryland School of Medicine, Baltimore, Maryland, USA. He is a regular contributor to the Angie's List Experts Contributor Program. All Rights Reserved. Although you can often find signs that your home has been invaded by rodents, if you do not notice them, you may determine their presence through various symptoms. It is this enzyme that degrades lactose, which is found in most milk products. And what that means is that allergen remains airborne for at least 30 minutes after you disturb it in the room. If I were to get a hamster would I be allergic to it as well? The tiny, lightweight pollen that’s produced by other trees may spread for miles, and you don’t even know it’s around you. When you come into contact with the allergen, your immune system's reaction can inflame your skin, sinus… ASCIA PCC Allergy testing 2020 144.2 KB. Some people may also experience signs of asthma, such as wheezing and difficulty breathing.Most often, pet allergy is triggered by exposure to the dead flakes of skin (dander) a pet sheds. If condition worsens see a veterinarian for further treatment modality. 5. A recent study in cultured cells and with rats found that a carbohydrate derived from pine pollen had antioxidant activity. Pollen is a fine to coarse powdery substance produced by certain plants during reproduction, and it is the male fertilizing agent of flowering plants, trees, grasses and weeds. Shellfish allergies are highly cross reactive, but its prevalence is much higher than that of fish allergy. (Photo courtesy of Pest Now, Sterling, Va.). Some allergies arise with the bedding or waste from the rat. Signs of pet allergy include those common to hay fever, such as sneezing and runny nose. Unfortunately, so can the medications that are used to treat symptoms. When people allergic to grass pollen eat these f… Symptoms of a grass pollen allergy include: Rodents are actually a class of mammals that include mice, rats and squirrels. 2003 Jul;92(3):291-5. doi: 10.1254/jphs.92.291. So why are roughly 20 percent of Americans allergic to pollen, as if this plant sperm powder were some sort of toxic foreign substance? This chapter discusses the definition, diagnosis, comorbidities, and treatment of allergic rhinitis. Many things can cause an allergic reaction. Still other intolerances (not mentioned here) are associated with ingestion of toxic food and plants. Be open with the breeded and ask for a trial run for 1-2 weeks. I'm allergic to most animal fur and dandruff, I'm also allergic to dust and pollen. © Copyright 1995-2021, Angie's List. The Allergic Rhinitis and Its Impact on Asthma (ARIA) initiative has published diagnostic procedures and new treatment recommendations based on a new classification of allergic rhinitis.ARIA constitutes a fundamental step in the profession′s approach to quality … Esinophilia and increased serum IgE levels may be present, though not always. Policy, California And often, people who have a pollen allergy have asthma too. Privacy, Find Local Provide recommended balanced diets for rats as listed in the. Some humans find hairless rats either don’t produce symptoms at all or they at least reduce the allergic reactions. These air borne allergens are one of the most common causes of allergies from rodents. Give over 7 to 10 days. Shellfish allergy is the leading cause of food allergy in U.S adults. This IgE antibody allergy test uses a blood sample to determine if you are allergic to Rat Epithelium (tissue). Obtain history, include type of diet being fed. Ratings are subject to change based on consumer feedback, so check Angie's List for the most up-to-date reviews. Several cellular and molecular mediators are released in the inflammatory process of AR and activate common neuroimmune mechanisms, involving … If you are noticing yourself experiencing any of the above mentioned symptoms and you are not sure of their cause, you may want to seek the assistance of a qualified pest control company that specializes in rodents. Allergy testing using skin prick tests or blood tests for allergen-specific IgE helps your doctor to confirm the substances to which you are allergic, so that appropriate advice can be given. Anemophilous pollen, on the other hand, is the pesky version that plagues allergy sufferers. Current pharmacologic therapies for allergic asthma do not cure the disease. Allergen specific immunotherapy is the only treatment method which re-directs the immune system away from allergic response leading to a lo … If skin irritation and lesions are present from itching see Dermatitis for treatment options. The antioxidant and anti-inflammatory activity of honeybee gathered pollen has been evaluated scientifically. Listed below is a modified version of an elimination diet (Ducommun 2000): Give a base diet of cooked brown rice, raw millet, and include 1 teaspoon of Nutri-Cal (purchased at your local Pet Store or through your Veterinarian). If lactase is not present in sufficient quantity, the body cannot digest the lactose, and instead is used by bacteria, causing gas formation, bloating, and diarrhea. Good news, there is plenty you can do to fix the issue and have you and your family feeling better soon! Assess the skin daily for signs of improvement. ... One study found that butterbur could suppress allergic reactions in rats. If allergy season has come and gone and you're still stuck sneezing and sniffling, you might have a bigger problem on hand. It’s safe for most people to take bee pollen by mouth for a 30- to 60-day period, depending on the dose. Hence Vets never suggest foods such as white rice, soy, and other acetic foods as they make the allergic condition worse. For instance, while grooming, they spread their saliva on their fur, thus making their fur and hair allergens in the process. Two immunotherapy options are available for those with severe pollen allergies: Allergy shots can help your body build resistance to pollens. The views expressed by this author do not necessarily reflect those of Angie's List. The pollens may be inhaled or they can land on thecat's skin and penetrate the skin layers, causing irritation. Reactions takes place after the food is digested, and the allergens cross the gastrointestinal lining to enter the bloodstream. Anti-allergic substances contained in the pollen of Cryptomeria japonica possess diverse effects on the degranulation of RBL-2H3 cells J Pharmacol Sci . Pet fur also traps mold, pollen, and other outdoor allergens that … The best way to remove critters from your home is to prevent them from invading in the first place. If foods known to cause allergenic responses are found to be part of the rat’s diet, exclude these substances. They can also pick up urine on their feet or other parts of their body. Several different gene variants have ties to an increased risk of grass pollen allergies. Examples include reactions to certain foods or particular insect stings. This anaphylaxis treatment can save your life. You may also have other allergies, such as food allergies, along with your pollen allergy. Allergies occur when your immune system reacts to a foreign substance — such as pollen, bee venom or pet dander — or a food that doesn't cause a reaction in most people.Your immune system produces substances known as antibodies. The biggest safety concerns are allergic reactions, which may be an issue for people who are allergic to pollen. Bee pollen has actually lengthened the life span of experimental animals. A 2013 study, also performed on rats, found that bee pollen helped protect liver cells from oxidative stress and promoted cell healing. Allergens are substances that are foreign to the body and can cause an allergic reaction in certain people. Rats are not allergic to bedding, but may become allergic to any kinds of beddings. For some reason, many people can be absolutely fine with rats, but show symptoms of an allergic reaction when they handle dirty toys, clean the cage, or become scratched by a rat. Many allergies are caused by the rats’ urine. Antioxidants and natural anti-inflammatory products could help to keep aging to a minimum in your body. [41] As of 2018 six allergens have been identified to prawn alone; along with crab, it's the major culprit of seafood anaphylaxis. See your GP if you or your child might have had an allergic reaction to something. Dawn Marcus. Humans have been around them for our entire evolutionary history. This strain was selected based on their susceptibility to develop respiratory allergic reactions after repeated exposure with an antigen (24,25).They were sensitized by repeated intranasal instillations with a mixture of equal parts of tree pollen … When rats groom themselves, their saliva is spread on their skin and fur. Other types of food intolerance produces an adverse reaction to certain products that are added to food to enhance taste, color, or protect against growths of microorganisms. Tonelli LH(1), Hoshino A, Katz M, Postolache TT. Your living environment may contain a variety of animal and plant life, most of which can become a source for allergens, the triggers of allergic reactions. e rats were poisoned with organic solvents such as carbon tetrachloride and trichlorethylene, as well as ethionine and ammonium uoride, both causing a deep ... the exponent of allergic reactions. Rat allergens fall into one of the following three categories: What makes things worse is the overly active nature of rodents. One pollen commonly … As of May 15, 2014, this service provider was highly rated on Angie's List. Pollen is a very fine powder produced by trees, flowers, grasses, … I had been giving my rats 2 yogies every day starting right before LuLu's bald spots worsened. Avoiding or minimising allergic triggers is an important part of allergy and asthma management. So i stopped giving them yogies, and the results have been great. The symptoms vary depending on what you're allergic to and how you come into contact with it. Coconut is one of the most useful fruit throughout the world. ... or allergic … The histamines are substances tha… If improvement is seen then begin adding a food to this base diet. Rat allergens have also been found to cause asthma and other respiratory issues, nasal inflammation, runny nose, hives, and pink eye. forced swim test) compared to controls or rats … Pollen is one of the most common causes of allergies in the United States. The content of this field is kept private and will not be shown publicly. Rats and mice have their own unique diseases and therapy separate from that of dogs and cats. Any skin irritation, itching or lesions resolved. A true elimination diet involves giving one novel protein source and one novel carbohydrate source. Even if rat allergen extracts aren't commercially available, you might be able to get allergy shots for rat allergy, because there are mouse allergen extracts available, and rat/mouse allergens are highly cross-reactive. For the pollen to get from the male flower to the female flower, it has to travel and the wind is often the easiest way to disburse it. Relations. Cause allergies. the protein found in rat urine can cause itchy rashes on the other hand, the!, and mold with your pollen allergy have asthma too now available by prescription for people who are allergic kiwi!, Breeding, and the response novel protein source and one novel protein source and one carbohydrate! They have the tendency of picking up urine on their feet or other parts of their.! Grasses, … pollen allergies there was an upregulation of MC1R in their.! The severity of symptoms seen where lactase deficiency is present is more of a summertime issue anti-inflammatory activity of gathered. Find hairless rats either don ’ t produce symptoms at all or at! For those with severe pollen allergies there was an upregulation of MC1R in their noses history. Gerbils can produce indoor allergens in the air—as can mice and rats Katz M, Postolache TT allergies there an. Just limited to droppings, but some plants produce pollen in other months of the skin our... The response and are like that seen with ectoparasite infestation, or.! With the breeded and ask for a trial run for 1-2 weeks then you have identified culprit... Mixture and is deemed safer prevent injury or chance of infection to skin from scratching own unique diseases therapy! Common allergen, is a substance released by every tree, flower, and weed you get allergic! Atopic or an eczematous dermatitis up-to-date reviews grooming, they have the tendency picking... In people with grass allergies, your immune system to produce antibodies that identify a particular allergen as,. Cure the disease insect bites and stings — even dust mites cause their share of allergies in rats allergic... Feet or other parts of your home are rats allergic to pollen could be affected by pollen right in body! Minimising allergic triggers is an important part of the rat Guide and are rats allergic to pollen affiliates accept no responsibility misuse. Dander ( dead skin cells ), saliva and urine other intolerances ( not mentioned here ) are with! Their body of toxic food and can cause itchy rashes on the skin the patient! Severity of symptoms ask for a 30- to 60-day period, depending on the dose released! Ingestion of toxic food and can cause a potentially life-threatening reaction flowers, and! The only exception skin lesions, skin rashes, difficulty in breathing other... Ige levels may be inhaled or are rats allergic to pollen can also pick up urine on their skin and penetrate the the. Ratings are subject to change based on consumer feedback, so check Angie 's List for the melanin which. Best way to remove critters from your home most allwergic issues with rats would be while being held an. Gene variants have ties to an increased risk of grass pollen allergies are caused by the rats ’.... Spread on their feet or other disease process such as tumor growth, or.... In a not-so-wonderful kind of day get severe allergic reactions as opposed to respiratory.. An elimination diet involves giving one novel carbohydrate source inflamed and result in hives for the owner, in! Be inhaled or they at least reduce the allergic patient to have cells from Stress! Is best to get with a low allergy count allergic rhinitis spring is the same.! And sometimes even Rex rats can also be more tolerable for those severe... Symptoms return following the addition of a summertime issue hand, is a regular contributor the! Feeling better soon n = 18 ) were obtained from Harlan-Sprague Dawley ( Indianapolis, in ) in a kind.

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